This technique has achieved a lot of importance, mainly when referring to male infertility. More than 90% of fertilization is reached following this technique.
This procedure is performed when:
- A few number of sperm with normal motility and morphology.
- Problems when the spermatozoon is penetrating the egg.
- Failure of conventional IVF.
- Antisperm antibodies stopping fecundation.
- Failure of acrosome response of spermatozoon.
- A few number of eggs.
- A big quantity of spermatozoa with no mobility or anomalous ones.
To begin with this procedure, male and female gamete are collected. Ovarian stimulation with drug support is needed to obtain a big quantity of eggs, as it was said before when talking about ovarian stimulation. Ovarian Hyperstimulation Syndrome can arise in a very small proportion of women as a result of this external stimulation. It is due to the over-sensitivity of the ovaries to the fertility drugs in certain patients. In most cases the condition can be managed conservatively.
Eggs are obtained by follicular puncture performing an ultrasound scan. This is performed under intravenous sedation and will last 15 or 20 minutes. Problems caused by this technique are very rare.
LABORATOY TREATMENT: DAY 0
Following egg collection, mature eggs are specially treated to remove the cumulus cells surrounding them (cumulus ooephorus cells). This way, mature and quality of eggs is studied. Only mature eggs are injected. (Phase II).
Inmature eggs will be treated in vitro following our own techniques.
The male partner will be asked to provide a sperm sample; then, this sample is prepared in the laboratory to obtain a spermatozoon of the best quality. After that, it is injected directly into the egg under microscopic control (ICSI). One at a time.
This technique is performed using one inverted microscope with micromanipulators which allows observing the spermatozoon 6,600 times bigger (IMSI). The egg is held by a microtitration diluter whilst another one injects the spermatozoon inside the egg.
All this is known by the couple and they give their consent. You have at your disposal information sheets about all techniques. There are also sheets for the couple to give their consent and accept what they are asking for.
24 hours after follicular puncture, eggs are examined for signs of fertilisation. The couple is then informed about the number of obtained fertilised eggs or zygotes.
This is just an informative point about the number of prepared pre-embryos for transfer after 24 or 48 hours, because it is possible than no all zygotes reach the pre-embryo stage.
DAY 2 / DAY 3 (TRANSFERENCE)
Viable obtained pre-embryos are deposited into the patient’s womb after 48 or 72 hours following follicular puncture. The pre-embryo is there to develop naturally.
It is clinically proved that when 3 embryos are transferred the chances of achieving a pregnancy increase and multiple pregnancy risk lows. Our Clinic use to transfer between 1 and 3 enbryos. When transferring it is important to take into account some data for the number and embryos and pregnancy to be correct: patient age, quality of embryos and womb, patient diagnosis, possible risk between mother and baby and desires of the couple.
Excess pre-embryos are frozen. There is an informative and consent form for the couple. These frozen embryos belong to the couple for 5 years; after that period and if the couple does not ask for them, the Clinic can use these pre-embryos for embryo donation.